The Protein Profiling Company

We measure proteins at scale for teams working to

We measure many proteins at once in every sample

n = 120 (and counting)

APRIL

BAFF

BDNF

CCL1 (TCA3)

CCL2 (MCP-1)

CCL3 (MIP-1 alpha)

CCL4 (MIP-1 beta)

CCL5 (RANTES)

CCL7 (MCP-3)

CCL8 (MCP-2)

CCL11 (Eotaxin)

CCL13 (MCP-4)

CCL14 (HCC-1 / HCC-3)

CCL15 (MIP-1 delta)

CCL16 (HCC-4)

CCL17 (TARC)

CCL18 (PARC)

CCL19 (MIP-3 beta)

CCL20 (MIP-3 alpha)

CCL21 (6Ckine)

CCL22 (MDC)

CCL23 (CK beta 8-1)

CCL24 (Eotaxin-2)

CCL25 (TECK)

CCL26 (Eotaxin-3)

CCL27

CCL28

CD30

CD40L

CRP

CXCL1 (GRO alpha)

CXCL2 (GRO beta)

CXCL3 (GRO gamma)

CXCL4 (PF4)

CXCL5 (ENA-78)

CXCL6  (GCP-2)

CXCL7  (NAP-2)

CXCL9  (MIG)

CXCL10  (IP-10 / CRG-2)

CXCL11  (I-TAC)

CXCL12  (SDF-1 alpha)

CXCL12  (SDF-1 beta)

CXCL13  (BLC / BCA-1)

CXCL14  (BRAK)

CXCL16

CXCL17  (VCC-1)

EGF

FGF-2

Fractalkine  (CX3CL1)

G-CSF

GM-CSF

GFAP

Granzyme  B

HGF

ICAM-1  (CD54)

IFN-alpha

IFN-beta

IFN-gamma

IL-1  alpha

IL-1  beta

IL-1RA

IL-2

IL-2RA

IL-3

IL-4

IL-5

IL-6

IL-7

IL-8  (CXCL8)

IL-9

IL-10

IL-12  (p40)

IL-12  (p70)

IL-13

IL-15

IL-16  (LCF)

IL-17  / IL-17A

IL-17B

IL-17C

IL-17D

IL-17F

IL-18

IL-20

IL-21

IL-22

IL-23  (IL-23 p19 & IL-12 p40)

IL-27

IL-31

IL-35  (IL27B & IL-12 p35)

LIF

M-CSF

MIF

MMP-1

MMP-2

MMP-3

MMP-7

MMP-9

MMP-10

MMP-12

NGF  beta

PCT  (Procalcitonin)

PDGF-BB

PLGF-1  (PLGF / PGF)

SAA

SCF  (c-kit)

TGF  beta 1

Tie-2

TNF  alpha

TNF  beta (Lymphotoxin-alpha)

TNF  RI (TNF R1)

TNF  RII (TNF R2)

TNF  RIII (Lymphotoxin-beta)

TRAIL  (TNFSF10)

TSLP

TWEAK  (TNFSF12)

VCAM-1  (CD106)

VEGF-A

VEGF-C

VEGF-D

VEGFR-1/Flt-1

What would you measure?

OUR mission

Unleash the full potential of proteomics

Despite two decades of technological advances for measuring DNA & RNA, technologies for measuring proteins have yet to be scaled. Today, scientists continue to compromise the number of proteins they measure - be it in academia, biopharma, or the clinic - typically resorting to measuring a handful of proteins 1-by-1.

At nplex, we are building technology to measure more proteins at a time, at lower cost, by leveraging advances in nanotechnology, software, and an open-platform approach. Our technology has been designed from the ground up to ensure it can be deployed on equipment available in every reference lab and hospital in the world.

Our mission is to bring scalable protein measurements to the world. Today, we're aiming to revolutionize how drugs are developed, and the way diseases are diagnosed and treated. While lofty, we firmly believe that human diseases can be eradicated through earlier detection, using protein biomarkers discovered and measured by our technology.

Our Technology

Introducing the nELISA

The nELISA is a miniaturized and highly parallelized version of the classical sandwich ELISA immunoassay. The platform has been under development since 2014.

The nELISA relies on DNA nanotechnology to engineer the topology of micron-sized beads, such that only a unique sandwich antibody pair co-exists on each bead. Each bead is itself custom engineered to be color-coded, letting us decipher the identity of beads in the assay.

This approach to scaling the ELISA ensures that we retain the high sensitivity and specificity of the ELISA gold standard, while also letting us measure 100's (soon to be 1000's) of different proteins at once on our color-coded bead sets.

Benefits

We scaled the nELISA to measure 120 secreted proteins - so far. Our plan is to measure every known protein in the human proteome.

nELISA is scalable because it inherently avoids the problem of antibody cross-reactivity. nELISA retains the specificity of sandwich ELISA, but without any cross-talk.

Miniaturizing nELISA onto micron-sized beads allows us to overcome the historical economic barriers of antibody-based proteomics.

Partners

Investors

Publications

Press

"Our investment in nplex"
OUR TEAM

Milad Dagher, PhD
CEO & Co-Founder

Milad brings a decade of nanotechnology, photonics, and assay engineering experience. Seeking a scalable alternative to the classical sandwich ELISA, Milad developed the core nELISA technology platform over the course of his PhD in the Juncker Lab.

David Juncker, PhD
Scientific Advisor & Co-Founder

David is a Professor and the Chair of Biomedical Engineering at McGill University. A co-inventor of the nELISA, David brings 20 years of insights into the miniaturization of biotechnologies, with a focus on biological applications and the use of protein-based assays for early disease diagnosis.

Jeff Munzar, PhD
Head of Technology & Co-Founder

Jeff brings deep DNA nanotechnology, molecular biology and broad software capabilities to the team. During his PhD in the Juncker Lab, Jeff used DNA nanotechnology to develop the first high throughput screening platform for dynamic aptamer biosensors.

Arya Tavakoli, MEng
Head of Laboratory Operations & Co-Founder

Arya is an aeronautical, now turned biomedical, engineer. Arya brings a half decade of project management experience from his time working to launch the CSeries aircraft at Bombardier, and experience with microfluidic point-of-care serological assays for vaccine efficacy studies.

Jinglin Kong, PhD
Head of Research and Development

Jinglin's passion lies in developing new biosensing tools, and she has experience taking them from idea to deployment. During her PhD at Cambridge, Jinglin used nanopores and DNA nanotechnology to develop an ultrasensitive single-molecule biosensing platform.

Woojong Rho, MSc
Assay Analyst

Woojong has expertise integrating biological assays to study protein markers of neurological diseases. During his Masters , Woojong optimized immunoassay platforms, including the nELISA, to measure proteins quantitatively for a range of human sample types.

JOIN US!

We're a tight-knit team working together to overcome hard technical and economical barriers and make massively scalable antibody-based proteomics a reality. We're growing fast and looking for talented and energetic teammates. Up for the challenge?  See our current opportunities!